Genetic immunization of ducks for production of antibodies specific to Helicobacter pylori UreB in egg yolks.
نویسندگان
چکیده
Following genetic immunization of laying ducks with a plasmid expressing Helicobacter pylori UreB (large subunit of urease), IgY against UreB were obtained from egg yolks. These polyclonal and monospecific IgY antibodies are of higher-titer and specifically recognize recombinant H. pylori urease purified from Escherichia coli. To our knowledge this is the first report describing generation of IgY antibodies directed against antigens of H. pylori by DNA-based immunization.
منابع مشابه
Development of anti-Helicobacter pylori immunoglobulins Y (IgYs) in quail
Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic bacterium that cause the stomach infection in more than 50% of human population worldwide. The aim of this study was to examine the possibility of anti-H. pylori immunoglobulins Y (IgYs) production in quails and evaluate the effect of the different methods of immunization on titers of IgY in egg yolks. Whole cell bacterial anti...
متن کاملProduction of specific IgY Helicobacter pylori recombinant OipA protein and assessment of its inhibitory effects towards attachment of H. pylori to AGS cell line
PURPOSE The common triple therapy for Helicobacter pylori is challenged by the increasing cases of antibiotic resistant infections, raising the need to explore alternative therapies. Oral administration of egg yolk immunoglobulin Y (IgY) has been previously reported as a means of passive immunization therapy for H. pylori infections. In this work, we investigated the inhibitory effect of IgY on...
متن کاملDevelopment of anti-Helicobacter pylori immunoglobulins Y (IgYs) in quail.
Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic bacterium that cause the stomach infection in more than 50% of human population worldwide. The aim of this study was to examine the possibility of anti-H. pylori immunoglobulins Y (IgYs) production in quails and evaluate the effect of the different methods of immunization on titers of IgY in egg yolks. Whole cell bacterial anti...
متن کاملOral immunization of mice with vaccine of attenuated Salmonella typhimurium expressing Helicobacter pylori urease B subunit.
OBJECTIVE To prepare the live recombinant vaccine of attenuated Salmonella typhimurium SL3261 expressing Helicobacter pylori (H. pylori) B subunit (UreB) and to determine whether it could be used as an oral vaccine against H. pylori infection. METHODS Using genomic DNA of H. pylori Sydney strain (SSI) as template, the H. pylori UreB gene fragment was amplified by PCR and subcloned into the ex...
متن کاملOral immunization of mice with lactic acid bacteria producing Helicobacter pylori urease B subunit partially protects against challenge with Helicobacter felis.
BACKGROUND The development of an efficacious vaccine against infection with Helicobacter pylori, the causative agent of chronic gastritis, peptic ulcer disease, and gastric adenocarcinoma, remains a challenge. Since the use of mucosal adjuvants is limited in human application, we have evaluated the potential of recombinant Lactobacillus strains producing H. pylori urease B (UreB) subunit to del...
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ورودعنوان ژورنال:
- Acta biochimica Polonica
دوره 52 1 شماره
صفحات -
تاریخ انتشار 2005